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Oriscience Cell Counting Kit-8 (CCK-8) 是一種基于WST-8的廣泛應(yīng)用于細(xì)胞增殖和細(xì)胞毒性檢測的快速、高靈敏度試劑盒。其原理為高度水溶性的四唑鹽WST-8在電子載體存在下，被細(xì)胞產(chǎn)生的脫氫酶還原產(chǎn)生水溶性甲臜染料，產(chǎn)生的甲臜量與活細(xì)胞的數(shù)量成正比。通過測量450 nm處的吸光度可間接反映活細(xì)胞的數(shù)量。

產(chǎn)品特色

操作簡單---即開即用，只需要一步可檢測結(jié)果

安全低毒---細(xì)胞毒性非常低，不影響后續(xù)實驗

結(jié)果可靠---靈敏度比MTT法更高，重現(xiàn)性好、穩(wěn)定性高

應(yīng)用性廣---細(xì)胞增殖測定、細(xì)胞毒性、藥物篩選等

儲存條件

     4℃避光保存

數(shù)據(jù)展示

A549細(xì)胞按500, 1000, 2000, 4000, 8000, 16000, 32000, 40000 cells接種至96孔板中，按10 μl/well加入Oriscience CCK-8與某日本公司CCK-8試劑孵育1 h后，測量450 nm處吸光度，計算R²值。結(jié)果顯示，Oriscience CCK-8具有更好的檢測靈敏度和更寬的線性范圍。

注意事項

1. 若細(xì)胞培養(yǎng)時間較長，建議不使用96孔板外周一圈，并在其中加入PBS以緩解蒸發(fā)，有助于提高實驗的均一性。

2. 本試劑盒的檢測依賴于脫氫酶催化的反應(yīng)，所以還原劑、抗氧化劑等物質(zhì)會干擾檢測，如果待測體系中存在較多此類物質(zhì)，須設(shè)法去除。

3. 檢測450 nm吸光度前需確保每個孔內(nèi)沒有氣泡，否則會干擾測定。

4. 本產(chǎn)品僅供科研使用。請勿用于醫(yī)藥、臨床診斷或治療，食品及化妝品等用途。

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